Comparing Fermentation Rate of the S. Cerevisiae Yeast in the Presence of Mgso4, Naf and Sodium Pyruvate

Syl Rogers Bio 210 Comparing Fermentation Rate of the S. cerevisiae Yeast in the presence of MgSO4, NaF and Sodium Pyruvate Hypothesis In the fermentation of rate of yeast, S. Cerevisiae, there will be a higher/ faster rate of ethanol production, However, using catalytic enzymes would make the rate more faster, and MgSo4 will have a higher rate of CO2 than that of NaF and Sodium pyruvate as it act as a more better catalytic enzyme than the others. Methods Preparation of Tubes A solution of yeast and glucose was prepared with different concentration of enzymes.In All the tubes water, 0. 3M glucose and yeast was placed; with the exception of some tubes in which 0. 2M MgSo4 and 2. 5mL and 1. 4ML 0. 2 NaF was placed respectively. In another tube was placed both MgSO4 and NaF. The different solutions were prepared and placed in separate tubes for the experiment. A 45oC water bath was setup to be used for the fermentation process. Data acquiring The tubes were placed in the water bath. A s top watch was used which aids in determining the reaction time.After Every 15 min, data was collected from all tubes by measuring the amount of CO2 being produced. This process continued for a total of 75min allowing enough time for Fermentation to occur in all tubes. Result For the first 15min, In the tube containing 8. 5mL water and 2. 5mL yeast only, the rate of the reaction was 0mm/min whiles the tube with the glucose has a rate of 233. 864mm/min. This is followed by the tube containing 2. 5mL of MgSo4 which has a rate of 165. 8573mm/min. The tube containing the MgSo4 and NaF has a rate of 69. 5mm/min which is followed by the 1. 24mL NaF tube that has a rate of 40. 63mm/min followed by 2. 5mL NaF tube which has a rate of 31. 08mm/min which was the lowest. After 30minutes, the tube containing only water and yeast has a rate of 0mm/min whiles the tube with the glucose has a rate of 208. 97mm/min. This is followed by the tube containing the MgSo4 which has a rate of 174. 1137mm/min . The tube containing the MgSo4 and NaF increased to a rate of 169. 59mm/min which is followed by the 1. 24mL NaF tube that has a rate of 57. 77mm/min followed by 2. mL NaF tube which has a rate of 35. 08mm/min which was the lowest. After an hour of reaction time, the MgSo4 and NaF tube has increased to a rate of 193. 17mm/min which is followed by the glucose tube with a rate CO2 rate of 176. 52mm/min followed by the MgSO4 Tube with a rate of 171. 73mm/min. The 1. 4mL NaF tube has increased to 61. 68mm/min followed by the 2. 4mL tube which was the lowest with a CO2 rate of 31. 31mm/min. For the final 15min of the experiment, the glucose tube has a rate of 176. 48mm/min followed by the MgSO4 and NaF tube with a rate of 169. 7mm/min. the MgSo4 tube has a rate of 157. 40mm/min which is followed by the 1. 24NaF tube with a rate CO2 rate of 57. 46mm/min followed by the 2. 5mL Tube with a rate of 29. 34mm/min which was the lowest CO2 production rate. Fig 1: Rate of CO2 production in the f ermentation of Glucose by S. cereviae Discussion: The fermentation of glucose by S. cerevisiae, which yields equal molar amounts of carbon dioxide and ethanol, showed higher production levels of carbon dioxideunder controlled conditions than any other implying the same for production of ethanol.Fermentation affected by a magnesium sulfate solution ranked second in carbon dioxideproduction while sodium fluoride yielded the least amount of carbon dioxide. These results suggest that magnesium has a positive influence on the production rate of carbon dioxide during the fermentation process and that the control contained an amount of magnesium that was neutralized by the presence of the fluoride anion. Though mean production was lower for the magnesium sulfate solution than the ontrol, a few specific results overlapped suggesting the addition of magnesium sulfate had no affect for those instances. A lower mean value for the magnesium sulfate solution suggests either that the fermentation process was supersaturated by magnesium or that the sulfate anion adversely affected the production of carbon dioxide. Further experimentation should be performed to determine the affects of magnesium sulfate in comparison to other magnesium and non-magnesium containing salts such as calcium sulfate or magnesium chloride to determine which ion has the greatest affect.Unknown variables such as the age of the S. cerevisiae samples may have affected the outcome of this experiment. Verification of S. cerevisiae batch age would allow for additional experiments to determine whether magnesium supplementation affects yeast of a certain age differently than that of another. Additionally, the experimental test tube/vial container allowed for some of each solution to be pushed out of the experimental test tube as the level of carbon dioxide increased.Further experimentation should utilize a better method of measuring the production of the entire original solution, not a fraction of the soluti on as a function of time. An alternative would be to attach a balloon to the tip of the solution-filled test tube, measure the diameter of the balloon, let an hour elapse and then measure the diameter again to determine carbon dioxide gas production. Decreased carbon dioxide production levels for S. erevisiae in the presence of a sodium fluoride solution suggest that an absence of available magnesium during fermentation results in an inefficient fermentation process; some level of magnesium is required in order for the process of fermentation to operate at maximum efficiency. Additional magnesium does not guarantee a faster or more efficient means of fermentation/ethanol production and can have an adverse affect on the fermentation process.

Drug Use in Vietname

Drug use by the soldiers in the Vietnam War was very common. Drug use in the military has always been around, but in the Vietnam era it started to increase at an alarming rate. It is noted that there were many drugs used and abused during this time. These drugs include marijuana, cocaine, and heroine. Soldiers often used these drugs to kill the pain of many hardships such as injury during battle, loss of a fellow soldier or being away from home. Marijuana is legally considered a drug according to the federal Controlled Substances Act; however the use of it was treated differently from other drugs by American soldiers and military lawyers in Vietnam. (Brush) Marijuana was present in Vietnam long before the arrival of the Americans and the start of the war. Drug laws were poorly defined and the enforcement of these poorly defined laws had little priority in the Vietnamese criminal justice system. The Vietnamese Government had no control over marijuana and there was no central drug enforcement agency. According to a survey made in 1966 by the U. S. military command in the Saigon area showed that there were 29 fixed outlets for the purchase of marijuana. The use and culture of marijuana in Vietnam has often been considered part of the cultural regulations for use, sale, and protocol and there is no sense of illegal activity like there is in the U. S. Many American soldiers have noted that the Vietnamese people used marijuana freely and openly during the time. There are even stories of American soldiers smoking marijuana with the South Vietnamese civilians and soldiers (Brush). In 1963, soldiers started using marijuana during the advisory period of the war. This was the earliest noted time of drug use in the Vietnam War and began before the widespread use in the United States. As its popularity grew, Congressional investigations began. In 1967 an investigation by congress revealed 16 instances of marijuana use inside the Marine brig at Da Nang. The source was the Vietnamese who gave it to prisoners on working parties. It was first often distributed by the soldiers throwing it into passing vehicles in which prisoners were riding (Brush). Prisoners were not the only people eligible to get the ample drug. While driving through urban areas marines found marijuana easily from vendors selling it on the streets. Marines took the offense of marijuana more seriously than the army. While the marines were subjecting all marijuana users to Courts-Marshall, the army only subjected users and dealers of hard drugs to trial. Although the marines took a more harsh approach to the use of marijuana, it was a failure. By 1969 use of marijuana was no longer just a trend of rear area units, and a drug rehabilitation was established in Cua Viet. This quote from a senior officer shows the helplessness against the rising trend of drug use among soldiers. â€Å"I don't know what the solution is†¦. I don't know what the hell we are going to do. † (Brush) Marijuana was not the only drug problem during the Vietnam during the war. During 1970 and 1971, the media started to carry news of two new drug trends. The studies of the media showed a growing number of white, middle-class young people, in suburbs as well as inner cities, were said to be using or dealing heroin. And United States military personnel-primarily in Vietnam, but also at duty stations in the United States and throughout the world-were similarly said to be sniffing, smoking, or mainlining heroin in substantial numbers. (Brecher) The problem of drug use gained President Richard Nixon’s attention, and in 1971 he said this to Congress, â€Å"Even now, there are no precise national statistics on drug use and drug addiction in the United States, the rate at which drug use is increasing, or where and how this increase is taking place. Most of what we think we know is extrapolated from those few states and cities where the dimensions of the problem have forced closer attention, including the maintenance of statistics. † Also in 1971, anecdotal evidence revealed that heroin had become the must popular illicit drug among citizens and soldiers of the U. S. During the summer of 1971, the public was informed of the widespread heroin use and addiction among many of the United States armed forces in Vietnam. Public officials from the President of the United States announced that everything possible would be done to rehabilitate or cure addicted soldiers serving in the war. This shocking information about the drug addicted soldiers serving in the army started a massive rehabilitation plan. Richard Nixon released this plan to Congress in 1971 with this statement, â€Å"Rehabilitation: A New Priority†¦. I am asking the Congress for a total of $105 million in addition to funds already contained in my 1972 budget to be used solely for the treatment and rehabilitation of drug-addicted individuals†¦. The nature of drug addiction, and the peculiar aspects of the present problem as it involves veterans [of the Vietnam war], make it imperative that rehabilitation procedures be undertaken immediately. . . In order to expedite the rehabilitation program of Vietnam veterans, I have ordered the immediate establishment of †¦ immediate rehabilitation efforts to be taken in Vietnam†¦. The Department of Defense will provide rehabilitation programs to all servicemen being returned for discharge who want this help, and we will be requesting legislation to permit the military services to retain for treatment any individual due for discharge who is a narcotic addict. All of our servicemen must be accorded the right to rehabilitation. â€Å"

Report (not essay) Essay Example | Topics and Well Written Essays - 1500 words

Report (not ) - Essay Example In the same vein, Chancellor Gordon Brown made a promise that Britain would be a leader in tackling the issue on an international level. Emission of CO2 from various industries and motor vehicles is choking the environment and leading to the greenhouse effect or trapping of large amounts of heat that leads to an increase in the earth’s temperature. During the last century, it is believed that the surface of the earth has warmed up by 0.75 degrees C. Other than CO2 , gases like methane, nitrous oxide and water vapour also contribute to this hazard. The following chart shows the sector wise distribution of global emissions: The UNFCCC defines climate change as â€Å" a change of climate that is attributed to directly or indirectly to human activity that alters the composition of global atmosphere and which is in addition to natural climate variability observed over comaprable time periods.†[Gleneagles G8 Summit] Some analysts however feel that the noise created around global warming might be the handiwork of some individuals for gaining their own interests. The situation is not really as bad as it is projected and in case reports about global warming are believed, we might be going back to pre-industrialization era.. Richard Courtney [1999] feels that people advocating the global warming theory are some researchers who are in need of research grants and some organisations working in the field of environment and need donations. He is of the opinion that they are promoting an imaginary fear of a man-made phenomenon. By promoting fear, they are selling their goods and ideas to the government and general public. It is clear that industries that cater to a clean and pollution-free environment will benefit from the commotion created around global warming and climate change. As such, industries that would discourage the use of fossil fuels, conventional methods of

Howard Gardner's Multiple Intelligences Term Paper

Howard Gardner's Multiple Intelligences - Term Paper Example and multiplication tables without a deep understanding of the project. There have been mixed reactions to this theory. Experiments have revealed that there is high correlation between the two. Many scholars are in support of the suggestive approaches of the theory. Without claiming it to be the final list Gardner has made a list of eight basic types of intelligences. The actual list prepared by Gardner had seven different types of intelligences and later in the year 1999, he added a naturalist intelligence which makes it a list of eight. ... Presently the theory’s eight accepted types of intelligences are musical, intrapersonal, naturalistic, interpersonal, spatial, bodily-kinesthetic, logical-mathematical and linguistic. The areas like ability to visualize with the eye of one’s mind and spatial judgment are deal by spatial intelligence. Architects, artists and designer according to the theory are examples of people with such intelligence. Studies also suggest that people with this type of intelligence are very good with puzzles. The linguistic intelligence area deals with people with high intelligence in the fields of words, written or spoken, people who have a high intelligence of verbal linguistics show a potentially high capability with languages and words. Such people are relatively good with memorizing dates, words, telling stories, reading and writing when compared to others. In such cases people who possess linguistic intelligence learn better by listening lectures, reading, by debates and discussio ns and by taking notes. Foreign languages are easily learned and remembered by people with verbal linguistic intelligence, they are very good in understanding and manipulating structure and grammar. They also possess a very high verbal memory and are able to recollect very fast. Logical – mathematical intelligence, this area deals with numbers, abstractions, reasoning and logic. Though it is often said that people with such intelligence are good at games like chess, subjects like computer programming, mathematics and other numeric and logical activities, the theory emphasizes that this type of intelligence has to do with various abilities like, performing calculations that are complex, investigations and scientific thinking, recognition of abstract patterns and reasoning abilities. The theory

Hume on Suicide Essay Example | Topics and Well Written Essays - 1250 words

Hume on Suicide - Essay Example This paper will begin with the statement that life is the most mysterious thing that humans can face. It is given to us and we have no opportunity to choose to be born or not to be born. The question is if humans have a right to get rid of their life or if a woman, who gifted this life, can take it away? If a person kills him/herself, it is called a suicide, if someone takes the life of another person, it is called a murder. From day to day you can hear in the news about many cases of suicide among people of different age and the question is if these acts can be considered as a human’s right to be a master of his/her life, or as a crime. Nobody can state for sure, if a person have right to commit a suicide and to get rid of the dearest that was given to him. There is an philosophy that suicide is a method to demonstrate to the God the power of humans in a short and strict form. The issue is very controversial and was discussed by many famous philosophers. David Hume is among t hem. He devoted the whole essay â€Å"Of Suicide† to this issue. The reception of Hume's views The unique and controversial idea presented by Hume is that there is no person in this world who will commit suicide if he considers his life to be valuable. Thus, according to Hume, a person has a right to commit a suicide: â€Å"to restore men to their native liberty, by examining all the common arguments against suicide, and showing that, that action may be free from every imputation of guilt or blame; according to the sentiments of all the ancient philosophers† (Essays on suicide and the immortality of the soul. p.3). If to consider this statement from the point of view of social issues and values, the supporters of it can be accused in the misrepresentation of God and religion as well as in immorality. Children are taught in school that only morally weak individuals can commit suicide as suicide is the easiest way to solve the problem. â€Å"Hume’s recent critics read him differently, however, as if his observations concerning the fragility of human life are intended to show (positively) that we have a low value in God’s eyes† (Holden 2005, p.203). Hume states that there are several stages before committing a suicide. A person usually feels disorder and weakness, apathy and stupidity (Muller, 1997). These are signals for the mind that it is time to finish the life. Not all the people in the modern society would agree with Hume on the aspect. According to him, each person who has such problems is a potential suicide committer. Nobody will deny that we always can find a reason for stress and problems are waiting for us every second, thus it is a usual thing to feel such symptoms. Hume also underlined that changes that could occur in life, would bring the unexpected results for sure. Thus, the question is if the changes only push to suicide or vice versa. Let’s imagine person who is about to commit a suicide. He or she is i n the last stage and suddenly the fortune proposes him or her a present, winning the lottery. It is interesting whether this can serve as a reverse point for a person and if it can make him change his mind. Philosophy, religion and everyday life Holden states: †Hume presents arguments for the moral permissibility of suicide that could readily be generalized to license any action, murder not excepted. But no one thinks that murder is permissible, so we have a clear reduction of Hume’s reasoning† (Holden 2005, p.190). However, Hume does not consider a suicide to be a murder. According to Hume, it is unfair to consider the suicide to be a crime. He considered material and animal world and comes to the conclusion that all the things inevitably return to their basic state. This argument is also very controversial. Politicians and the law as well as religion dictate what the citizens should do and how they should behave in the society. In reality each country has its la w and unwritten rules of behavior, the picture of the world, which form the mentality (Flew, 1986).

Art Curation Research Paper Example | Topics and Well Written Essays - 2000 words

Art Curation - Research Paper Example With the accommodation of terms such as performative, or self-reflexive, it is clear that curating is no longer only about exhibitions. This form of discourse proves that curating is slowly turning into a visible, self-critical and transparent process. The rising transformation and visibility of the exhibition since the 20th century has encouraged students to develop a motivation for engaging with the history of curation. On one hand, the transformation narrows down to the foundation of new institutions that accommodate for the exhibition of contemporary art, increasing temporalisation of museums as well as expanding the art market with countless art fairs and gallery shows(Vogel, 2013)1. Besides the need to renovate museums and enlarge their temporary exhibition spaces for marketing and other economic-related reasons, an artistic engagement with the museum’s collection has become a critical requirement for everymuseum. Regardless of the fact that these strategies are based on permanent collections, the presentation approaches have replaced the atemporal collection displaythat is viewed as rigid and authoritative(Altshuler, 2012)2. On the other hand, the art exhibition is changing to an extent that it encourages us to appreciate a range of typologies that overshadow the conventional formats of solo, group or thematic shows. The discourse in art curation considers the growth of new artistic practices that include institutional critique or conceptual art(Bourriaud, 2002)3.

Punishment Term Paper Example | Topics and Well Written Essays - 1250 words

Punishment - Term Paper Example They can be fined, incarcerated or sentenced to death for the gravest offences in some countries such as the US, Japan, China and the Kingdom of Saudi Arabia among others. Corporal punishment is applied by teachers and parents in schools and in the family setting to encourage good behavior among children. The issue of punishment usually raises concerns in regard to the different understanding and application of punishment across different spheres in the society. Contentions arise in trying to determine the standards for determining the severity of a moral violation, and the punishment that fits it. This paper presents a critique of the moral principles that might be used to determine the match between moral violation and punishment. Hart postulated some elements that may be used to justify punishment. For example, he argues that the punishment has to be for an offence that is contrary to the set of laws in a particular state and also has to be enforced and undertaken by an authority that is defined by a legal system, which the offender is in breach of (qtd. in Sverdlik, 180). This indicates that the punishment should only be based on particular rules that have been set and therefore punishment for offences that are beyond the legally known rules is not justifiable. This argument leaves teachers and parents in a position whereby they are not justified in regard to the punishments that they give to children. They punish the children depending on their perception regarding what is right or wrong. The appropriateness of the punishment is usually not clear and the legality of punishments in schools as well as at home is disputable. The same case applies in the punishments meted out on individuals who engage in antis ocial behaviors in the society. For instance, mob justice is one of the punishments that the public resorts to after catching a suspected criminal. The punishment that